Comparing 2D PAGE Gels May Suggest Putative Spot Identification
- Scientists around the world often work on similar 2D gel data
- Many 2D gel databases and gel maps exist on the Web with
identified proteins
- Comparing 2D sample gels against these 2D gel database maps may
suggest putative protein spot identification
- How can we do this comparison?
Example: corresponding regions of plasma protein gels from different labs
ExPASy 2D gel Database Merril (NIMH)
Immobilized pH Gradient Carrier Ampholytes
Non-linear gradient Linear Gradient
http://www.expasy.ch/
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DEMO: Demo