BreastDB - Breast cancer drug resistance screen Database


[Under construction]

Table Of Contents

  1. Introduction to the BreastDB database
  2. Search the breast cancer drug screen database
  3. Clickable 2D gel map of identified MCF7 proteins
  4. Database definitions
  5. Flicker compare 2D gels from this database
  6. Search 2DWG gel image database for NCI breast tissue gels
  7. References

Introduction to the BreastDB Drug Resistance Screen database

This BreastDB database is derived from the NCI breast cancer drug resistance screen protein expression database [1-2] . That database is dedicated to the protein changes in the 60 human cancer cell lines.

This data has been placed here at an early stage of the analysis to illustrate its application in the database program and to indicate "coming attractions" from this database, not to tell the scientific story involving development of drug resistance. This database will be upgraded with more extensive data using more advance gel techniques.

The current MCF7 cell line variants are denoted L621B, L612C, L612E, L612J, L612L, L612N, L612P, L612Q, L612S, L614P, L614Q and L614S. They constitute the following samples:

  1. L621B - *** to be expanded ***
  2. L612C -
  3. L612E -
  4. L612J -
  5. L612L -
  6. L612N -
  7. L612P -
  8. L612Q -
  9. L612S -
  10. L614P -
  11. L614Q -
  12. L614S -

The BreastDB uses a simple database search engine dbEngine [3] (to access selected rows of gels in the full database table. The search expression is made up of combinations of words (i.e. terms) contained in the database. The search is conducted on each row with that row reported if a match is found.

It is possible to flicker compare [4] gel images from the different samples. This allows you to see subtle as well as gross changes in spot quantitation and position. You can Flicker compare 2D gels from this database.

The user is encouraged to contact the editor with corrections, or for preprints, reprints, and other information related to proteins to be included in this database.

Search the breast cancer drug resistance screen database

The database may be searched to find entries matching a key phrases in any of the data for that entry. Search the Breast Cancer Cell Line database by specifying search terms below (one per entry). Each entry is searched for the conjunction (i.e. AND) or disjunction (i.e. OR) of the terms. [Note terms may be any part of a database entry but may not include any spaces.]


Enter search terms (you may use either AND or OR term connectives):

If searching for "Beta Actin", use "Beta AND actin" since the "AND" is required because of the space. Search is case-independent. [Note: currently you can use either all AND connectives or all OR connectives - but not both.]

Eg. use "Hsp" to find all Heat shock proteins.
Eg. use "BC" to find all proteins in the BreastDB with BCxxxx id numbers.

References

  1. Weinstein JN, Myers TG, O'Connor PM, Friend SH, Fornace AJ Jr, Kohn KW, Fojo T, Bates SE, Rubinstein LV, Anderson NL, Buolamwini JK, van Osdol WW, Monks AP, Scudiero DA, Sausville EA, Zaharevitz DW, Bunow B, Viswanadhan VN, Johnson GS, Wittes RE, Paull KD (1997). An information-intensive approach to the molecular pharmacology of cancer. Science 275 343-349.

  2. Myers TG, Anderson NL, Waltham M, Li G, Buolamwini JK, Scudiero DA, Paull KD, Sausville EA, Weinstein JN (1997) A protein expression database for the molecular pharmacology of cancer. Electrophoresis 18(3-4) 647-653.

  3. Lemkin PF, Chipperfield M, Merril C, Zullo S (1996) A World Wide Web (WWW) server database engine for an organelle database, MitoDat. Electrophoresis 17(2), 556-572. (See dbEngine documentation)

  4. Lemkin PF (1997) Comparing Two-Dimensional electrophoretic gels across the Internet. Electrophoresis, 18, 461-470. (See Flicker Server)

Database Maintenance

  • Database files (restricted access)

    $Date: 1997/07/09 17:09:47 $ / $Revision: 1.24 $ /
    weinstein@dtpax2.ncifcrf.gov, J. Weinstein (editor)
    lemkin@ncifcrf.gov, P. Lemkin (Web), (LECB,NCI/FCRDC)