Flicker compare protein phosphorylation 2D studies in mouse T lymphocytes

This data is taken from the phosphoDB Phosphoprotein database which identifies some of the proteins in these gels. The two images demonstrate differences in protein phosphorylation at different stages of the cell cycle. CT.4R cells were labeled with 32P-orthophosphate prior to lysis and analysis as previously described. The first image (59g-PPDB-G1-phase) represents proteins from cells enriched in the G1-phase of the cell cycle, while the second image (59h-PPDB-G2/M phase) represents cells in late G2 and M.

See the Flicker Reference Manual for more details on running flicker. You can get data from that database from flicker if you 1) set the "Click to access DB" checkbox, and 2) click on a spot.

To navigate around an image, you can use the scroll bars and then click on the spot of interest. Hint: however, it is easier if you hold the CONTROL key and then click on a point in the image. This will center that point in the scrollable window. It will also display the (x,y) position of the cursor in that image at the top of that window. After you have done an initial alignment, you can move both images "ganged" together by clicking on a spot in either image with both the SHIFT and CONTROL keys pressed.

Click on the Flicker checkbox to turn flickering on or off. You align the images by navigating one of the two as in the previous step until you are satisfied with the alignment.

Left Image: lymphocyte-T_mouse_32P_59g-PPDB - G1-phase
Right Image: lymphocyte-T_mouse_32P_59h-PPDB - G2/M phase

Please WAIT while images load.

$Date: 1998/11/16 23:09:13 $ lemkin@ncifcrf.gov