3. Visually comparing 2D gels with image flickering

• Flickering is the rapid alternate display of two images which overlay the same visual space

• Images are locally aligned by moving one image with respect to the other one while flickering

• Images appear to fuse together if
  1. flicker rate is adjusted (0.1 to 1 second) for type of material and particular person viewing
  2. user is reasonably close to display
  3. local regions are well aligned with most features aligned

• Differential flicker (displaying one image longer than the other) is useful for comparing light and dark samples

• Mechanical flickering had been used for years in astromomy for comparing star maps. N.G. Anderson used it for comparing 2D gels

• We used computer flickering in GELLAB for
  1. finding spot differences
  2. aligning gels when landmarking spots for building multi-gel databases

 

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DEMO: Demo