9. Limitations with the Flicker method

• Similar data - comparisons are only potentially as good as the data which is being compared. I.e.
  1. use similar material
  2. prepared under similar conditions

• Spot identification - the comparisons will only give putative identifications but they may suggest antibodies to try without having to sequence the spots of interest

• pIe, MW, OD Calibration - the current design does not calibrate 2D gels in terms of pIe, molecular mass, and optical density (OD)

• Some users occasionally have problems visualizing changes or are bothered by flickering images

 

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DEMO: Demo